The Membrane Protein of Severe Acute Respiratory Syndrome Coronavirus Acts as a Dominant Immunogen Revealed by a Clustering Region of Novel Functionally and Structurally Defined Cytotoxic T-Lymphocyte Epitopes
Identifieur interne : 002915 ( Main/Exploration ); précédent : 002914; suivant : 002916The Membrane Protein of Severe Acute Respiratory Syndrome Coronavirus Acts as a Dominant Immunogen Revealed by a Clustering Region of Novel Functionally and Structurally Defined Cytotoxic T-Lymphocyte Epitopes
Auteurs : JUN LIU [République populaire de Chine] ; YEPING SUN [République populaire de Chine] ; JIANXUN QI [République populaire de Chine] ; FULIANG CHU [République populaire de Chine] ; HAO WU [République populaire de Chine] ; FENG GAO [République populaire de Chine] ; TAISHENG LI [République populaire de Chine] ; JINGHUA YAN [République populaire de Chine] ; George F. Gao [République populaire de Chine]Source :
- The Journal of infectious diseases [ 0022-1899 ] ; 2010.
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Abstract
Background. Severe acute respiratory syndrome coronavirus (SARS-CoV), which emerged with highly contagious and life-threatening characteristics in 2002, remains a potential risk for future outbreaks. Membrane (M) and envelope (E) proteins are major structural proteins of the SARS-CoV The M protein has been determined as a protective antigen in humoral responses. However, its potential roles in stimulating cellular immunity remain elusive. Methods. In this study, a panel of peptides derived from M and E proteins were tested by in vitro refolding, T2 cell-binding assays, and responses stimulated by cytotoxic T-lymphocyte (CTL) epitopes in HLA-A2.1/Kb transgenic mice and human peripheral blood mononuclear cells (PBMCs). Results. A nonameric epitope Mn2 and a decameric epitope Md3 derived from the M protein were identified and used for the evaluation of M protein-specific immunity. Responses stimulated by M protein-specific CTL epitopes have been found in the PBMCs of donors who had recovered from SARS infection. Additionally, the transmembrane domain of the M protein may contain a T cell epitope cluster revealed by the immunogenic and structural analysis of a panel of truncated peptides overlapping with Mn2 and Md3. Conclusions. The M protein of SARS-CoV holds dominant cellular immunogenicity. This, together with previous reports of a strong humoral response against the M protein, may help to further explain the immunogenicity of SARS and serves as potential targets for SARS-CoV vaccine design.
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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en" level="a">The Membrane Protein of Severe Acute Respiratory Syndrome Coronavirus Acts as a Dominant Immunogen Revealed by a Clustering Region of Novel Functionally and Structurally Defined Cytotoxic T-Lymphocyte Epitopes</title><author><name sortKey="Jun Liu" sort="Jun Liu" uniqKey="Jun Liu" last="Jun Liu">JUN LIU</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences (CAS)</s1><s3>CHN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ></inist:fA14><country>République populaire de Chine</country><wicri:noRegion>CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences (CAS)</wicri:noRegion></affiliation><affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Graduate University, CAS</s1><s3>CHN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>9 aut.</sZ></inist:fA14><country>République populaire de Chine</country><wicri:noRegion>Graduate University, CAS</wicri:noRegion></affiliation><affiliation wicri:level="1"><inist:fA14 i1="03"><s1>China-Japan Joint Laboratory of Molecular Immunology and Molecular Microbiology, Institute of Microbiology, CAS</s1><s3>CHN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ></inist:fA14><country>République populaire de Chine</country><wicri:noRegion>China-Japan Joint Laboratory of Molecular Immunology and Molecular Microbiology, Institute of Microbiology, CAS</wicri:noRegion></affiliation></author><author><name sortKey="Yeping Sun" sort="Yeping Sun" uniqKey="Yeping Sun" last="Yeping Sun">YEPING SUN</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences (CAS)</s1><s3>CHN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ></inist:fA14><country>République populaire de Chine</country><wicri:noRegion>CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences (CAS)</wicri:noRegion></affiliation><affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Graduate University, CAS</s1><s3>CHN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>9 aut.</sZ></inist:fA14><country>République populaire de Chine</country><wicri:noRegion>Graduate University, CAS</wicri:noRegion></affiliation><affiliation wicri:level="1"><inist:fA14 i1="03"><s1>China-Japan Joint Laboratory of Molecular Immunology and Molecular Microbiology, Institute of Microbiology, CAS</s1><s3>CHN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ></inist:fA14><country>République populaire de Chine</country><wicri:noRegion>China-Japan Joint Laboratory of Molecular Immunology and Molecular Microbiology, Institute of Microbiology, CAS</wicri:noRegion></affiliation></author><author><name sortKey="Jianxun Qi" sort="Jianxun Qi" uniqKey="Jianxun Qi" last="Jianxun Qi">JIANXUN QI</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences (CAS)</s1><s3>CHN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ></inist:fA14><country>République populaire de Chine</country><wicri:noRegion>CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences (CAS)</wicri:noRegion></affiliation><affiliation wicri:level="1"><inist:fA14 i1="03"><s1>China-Japan Joint Laboratory of Molecular Immunology and Molecular Microbiology, Institute of Microbiology, CAS</s1><s3>CHN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ></inist:fA14><country>République populaire de Chine</country><wicri:noRegion>China-Japan Joint Laboratory of Molecular Immunology and Molecular Microbiology, Institute of Microbiology, CAS</wicri:noRegion></affiliation></author><author><name sortKey="Fuliang Chu" sort="Fuliang Chu" uniqKey="Fuliang Chu" last="Fuliang Chu">FULIANG CHU</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences (CAS)</s1><s3>CHN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ></inist:fA14><country>République populaire de Chine</country><wicri:noRegion>CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences (CAS)</wicri:noRegion></affiliation></author><author><name sortKey="Hao Wu" sort="Hao Wu" uniqKey="Hao Wu" last="Hao Wu">HAO WU</name><affiliation wicri:level="1"><inist:fA14 i1="04"><s1>Department of Infectious Diseases, You-An Hospital, Capital Medical University</s1><s3>CHN</s3><sZ>5 aut.</sZ></inist:fA14><country>République populaire de Chine</country><wicri:noRegion>Department of Infectious Diseases, You-An Hospital, Capital Medical University</wicri:noRegion></affiliation></author><author><name sortKey="Feng Gao" sort="Feng Gao" uniqKey="Feng Gao" last="Feng Gao">FENG GAO</name><affiliation wicri:level="1"><inist:fA14 i1="05"><s1>Institute of Biophysics, CAS</s1><s3>CHN</s3><sZ>6 aut.</sZ></inist:fA14><country>République populaire de Chine</country><wicri:noRegion>Institute of Biophysics, CAS</wicri:noRegion></affiliation></author><author><name sortKey="Taisheng Li" sort="Taisheng Li" uniqKey="Taisheng Li" last="Taisheng Li">TAISHENG LI</name><affiliation wicri:level="1"><inist:fA14 i1="06"><s1>Department of Infectious Diseases, Peking Union Medical College Hospital and AIDS Research Center, Chinese Academy of Medical Sciences and Peking Union Medical College</s1><s3>CHN</s3><sZ>7 aut.</sZ></inist:fA14><country>République populaire de Chine</country><wicri:noRegion>Department of Infectious Diseases, Peking Union Medical College Hospital and AIDS Research Center, Chinese Academy of Medical Sciences and Peking Union Medical College</wicri:noRegion></affiliation></author><author><name sortKey="Jinghua Yan" sort="Jinghua Yan" uniqKey="Jinghua Yan" last="Jinghua Yan">JINGHUA YAN</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences (CAS)</s1><s3>CHN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ></inist:fA14><country>République populaire de Chine</country><wicri:noRegion>CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences (CAS)</wicri:noRegion></affiliation><affiliation wicri:level="1"><inist:fA14 i1="03"><s1>China-Japan Joint Laboratory of Molecular Immunology and Molecular Microbiology, Institute of Microbiology, CAS</s1><s3>CHN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ></inist:fA14><country>République populaire de Chine</country><wicri:noRegion>China-Japan Joint Laboratory of Molecular Immunology and Molecular Microbiology, Institute of Microbiology, CAS</wicri:noRegion></affiliation></author><author><name sortKey="Gao, George F" sort="Gao, George F" uniqKey="Gao G" first="George F." last="Gao">George F. Gao</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences (CAS)</s1><s3>CHN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ></inist:fA14><country>République populaire de Chine</country><wicri:noRegion>CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences (CAS)</wicri:noRegion></affiliation><affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Graduate University, CAS</s1><s3>CHN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>9 aut.</sZ></inist:fA14><country>République populaire de Chine</country><wicri:noRegion>Graduate University, CAS</wicri:noRegion></affiliation><affiliation wicri:level="1"><inist:fA14 i1="03"><s1>China-Japan Joint Laboratory of Molecular Immunology and Molecular Microbiology, Institute of Microbiology, CAS</s1><s3>CHN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ></inist:fA14><country>République populaire de Chine</country><wicri:noRegion>China-Japan Joint Laboratory of Molecular Immunology and Molecular Microbiology, Institute of Microbiology, CAS</wicri:noRegion></affiliation><affiliation wicri:level="3"><inist:fA14 i1="07"><s1>Beijing Institutes of Life Science, CAS</s1><s2>Beijing</s2><s3>CHN</s3><sZ>9 aut.</sZ></inist:fA14><country>République populaire de Chine</country><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">INIST</idno><idno type="inist">10-0482639</idno><date when="2010">2010</date><idno type="stanalyst">PASCAL 10-0482639 INIST</idno><idno type="RBID">Pascal:10-0482639</idno><idno type="wicri:Area/PascalFrancis/Corpus">000132</idno><idno type="wicri:Area/PascalFrancis/Curation">000856</idno><idno type="wicri:Area/PascalFrancis/Checkpoint">000123</idno><idno type="wicri:explorRef" wicri:stream="PascalFrancis" wicri:step="Checkpoint">000123</idno><idno type="wicri:doubleKey">0022-1899:2010:Jun Liu:the:membrane:protein</idno><idno type="wicri:Area/Main/Merge">002955</idno><idno type="wicri:Area/Main/Curation">002915</idno><idno type="wicri:Area/Main/Exploration">002915</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a">The Membrane Protein of Severe Acute Respiratory Syndrome Coronavirus Acts as a Dominant Immunogen Revealed by a Clustering Region of Novel Functionally and Structurally Defined Cytotoxic T-Lymphocyte Epitopes</title><author><name sortKey="Jun Liu" sort="Jun Liu" uniqKey="Jun Liu" last="Jun Liu">JUN LIU</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences (CAS)</s1><s3>CHN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ></inist:fA14><country>République populaire de Chine</country><wicri:noRegion>CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences (CAS)</wicri:noRegion></affiliation><affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Graduate University, CAS</s1><s3>CHN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>9 aut.</sZ></inist:fA14><country>République populaire de Chine</country><wicri:noRegion>Graduate University, CAS</wicri:noRegion></affiliation><affiliation wicri:level="1"><inist:fA14 i1="03"><s1>China-Japan Joint Laboratory of Molecular Immunology and Molecular Microbiology, Institute of Microbiology, CAS</s1><s3>CHN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ></inist:fA14><country>République populaire de Chine</country><wicri:noRegion>China-Japan Joint Laboratory of Molecular Immunology and Molecular Microbiology, Institute of Microbiology, CAS</wicri:noRegion></affiliation></author><author><name sortKey="Yeping Sun" sort="Yeping Sun" uniqKey="Yeping Sun" last="Yeping Sun">YEPING SUN</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences (CAS)</s1><s3>CHN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ></inist:fA14><country>République populaire de Chine</country><wicri:noRegion>CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences (CAS)</wicri:noRegion></affiliation><affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Graduate University, CAS</s1><s3>CHN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>9 aut.</sZ></inist:fA14><country>République populaire de Chine</country><wicri:noRegion>Graduate University, CAS</wicri:noRegion></affiliation><affiliation wicri:level="1"><inist:fA14 i1="03"><s1>China-Japan Joint Laboratory of Molecular Immunology and Molecular Microbiology, Institute of Microbiology, CAS</s1><s3>CHN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ></inist:fA14><country>République populaire de Chine</country><wicri:noRegion>China-Japan Joint Laboratory of Molecular Immunology and Molecular Microbiology, Institute of Microbiology, CAS</wicri:noRegion></affiliation></author><author><name sortKey="Jianxun Qi" sort="Jianxun Qi" uniqKey="Jianxun Qi" last="Jianxun Qi">JIANXUN QI</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences (CAS)</s1><s3>CHN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ></inist:fA14><country>République populaire de Chine</country><wicri:noRegion>CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences (CAS)</wicri:noRegion></affiliation><affiliation wicri:level="1"><inist:fA14 i1="03"><s1>China-Japan Joint Laboratory of Molecular Immunology and Molecular Microbiology, Institute of Microbiology, CAS</s1><s3>CHN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ></inist:fA14><country>République populaire de Chine</country><wicri:noRegion>China-Japan Joint Laboratory of Molecular Immunology and Molecular Microbiology, Institute of Microbiology, CAS</wicri:noRegion></affiliation></author><author><name sortKey="Fuliang Chu" sort="Fuliang Chu" uniqKey="Fuliang Chu" last="Fuliang Chu">FULIANG CHU</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences (CAS)</s1><s3>CHN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ></inist:fA14><country>République populaire de Chine</country><wicri:noRegion>CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences (CAS)</wicri:noRegion></affiliation></author><author><name sortKey="Hao Wu" sort="Hao Wu" uniqKey="Hao Wu" last="Hao Wu">HAO WU</name><affiliation wicri:level="1"><inist:fA14 i1="04"><s1>Department of Infectious Diseases, You-An Hospital, Capital Medical University</s1><s3>CHN</s3><sZ>5 aut.</sZ></inist:fA14><country>République populaire de Chine</country><wicri:noRegion>Department of Infectious Diseases, You-An Hospital, Capital Medical University</wicri:noRegion></affiliation></author><author><name sortKey="Feng Gao" sort="Feng Gao" uniqKey="Feng Gao" last="Feng Gao">FENG GAO</name><affiliation wicri:level="1"><inist:fA14 i1="05"><s1>Institute of Biophysics, CAS</s1><s3>CHN</s3><sZ>6 aut.</sZ></inist:fA14><country>République populaire de Chine</country><wicri:noRegion>Institute of Biophysics, CAS</wicri:noRegion></affiliation></author><author><name sortKey="Taisheng Li" sort="Taisheng Li" uniqKey="Taisheng Li" last="Taisheng Li">TAISHENG LI</name><affiliation wicri:level="1"><inist:fA14 i1="06"><s1>Department of Infectious Diseases, Peking Union Medical College Hospital and AIDS Research Center, Chinese Academy of Medical Sciences and Peking Union Medical College</s1><s3>CHN</s3><sZ>7 aut.</sZ></inist:fA14><country>République populaire de Chine</country><wicri:noRegion>Department of Infectious Diseases, Peking Union Medical College Hospital and AIDS Research Center, Chinese Academy of Medical Sciences and Peking Union Medical College</wicri:noRegion></affiliation></author><author><name sortKey="Jinghua Yan" sort="Jinghua Yan" uniqKey="Jinghua Yan" last="Jinghua Yan">JINGHUA YAN</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences (CAS)</s1><s3>CHN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ></inist:fA14><country>République populaire de Chine</country><wicri:noRegion>CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences (CAS)</wicri:noRegion></affiliation><affiliation wicri:level="1"><inist:fA14 i1="03"><s1>China-Japan Joint Laboratory of Molecular Immunology and Molecular Microbiology, Institute of Microbiology, CAS</s1><s3>CHN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ></inist:fA14><country>République populaire de Chine</country><wicri:noRegion>China-Japan Joint Laboratory of Molecular Immunology and Molecular Microbiology, Institute of Microbiology, CAS</wicri:noRegion></affiliation></author><author><name sortKey="Gao, George F" sort="Gao, George F" uniqKey="Gao G" first="George F." last="Gao">George F. Gao</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences (CAS)</s1><s3>CHN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ></inist:fA14><country>République populaire de Chine</country><wicri:noRegion>CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences (CAS)</wicri:noRegion></affiliation><affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Graduate University, CAS</s1><s3>CHN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>9 aut.</sZ></inist:fA14><country>République populaire de Chine</country><wicri:noRegion>Graduate University, CAS</wicri:noRegion></affiliation><affiliation wicri:level="1"><inist:fA14 i1="03"><s1>China-Japan Joint Laboratory of Molecular Immunology and Molecular Microbiology, Institute of Microbiology, CAS</s1><s3>CHN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ></inist:fA14><country>République populaire de Chine</country><wicri:noRegion>China-Japan Joint Laboratory of Molecular Immunology and Molecular Microbiology, Institute of Microbiology, CAS</wicri:noRegion></affiliation><affiliation wicri:level="3"><inist:fA14 i1="07"><s1>Beijing Institutes of Life Science, CAS</s1><s2>Beijing</s2><s3>CHN</s3><sZ>9 aut.</sZ></inist:fA14><country>République populaire de Chine</country><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author></analytic><series><title level="j" type="main">The Journal of infectious diseases</title><title level="j" type="abbreviated">J. infect. dis.</title><idno type="ISSN">0022-1899</idno><imprint><date when="2010">2010</date></imprint></series></biblStruct></sourceDesc><seriesStmt><title level="j" type="main">The Journal of infectious diseases</title><title level="j" type="abbreviated">J. infect. dis.</title><idno type="ISSN">0022-1899</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Antigenic determinant</term><term>Coronavirus</term><term>Cytotoxic T lymphocyte</term><term>Cytotoxicity</term><term>Infection</term><term>Membrane protein</term><term>Severe acute respiratory syndrome</term><term>T-Lymphocyte</term></keywords><keywords scheme="Pascal" xml:lang="fr"><term>Coronavirus</term><term>Protéine membranaire</term><term>Cytotoxicité</term><term>Lymphocyte T cytotoxique</term><term>Lymphocyte T</term><term>Déterminant antigénique</term><term>Infection</term><term>Syndrome respiratoire aigu sévère</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Background. Severe acute respiratory syndrome coronavirus (SARS-CoV), which emerged with highly contagious and life-threatening characteristics in 2002, remains a potential risk for future outbreaks. Membrane (M) and envelope (E) proteins are major structural proteins of the SARS-CoV The M protein has been determined as a protective antigen in humoral responses. However, its potential roles in stimulating cellular immunity remain elusive. Methods. In this study, a panel of peptides derived from M and E proteins were tested by in vitro refolding, T2 cell-binding assays, and responses stimulated by cytotoxic T-lymphocyte (CTL) epitopes in HLA-A2.1/K<sup>b</sup> transgenic mice and human peripheral blood mononuclear cells (PBMCs). Results. A nonameric epitope Mn2 and a decameric epitope Md3 derived from the M protein were identified and used for the evaluation of M protein-specific immunity. Responses stimulated by M protein-specific CTL epitopes have been found in the PBMCs of donors who had recovered from SARS infection. Additionally, the transmembrane domain of the M protein may contain a T cell epitope cluster revealed by the immunogenic and structural analysis of a panel of truncated peptides overlapping with Mn2 and Md3. Conclusions. The M protein of SARS-CoV holds dominant cellular immunogenicity. This, together with previous reports of a strong humoral response against the M protein, may help to further explain the immunogenicity of SARS and serves as potential targets for SARS-CoV vaccine design.</div></front></TEI><affiliations><list><country><li>République populaire de Chine</li></country><settlement><li>Pékin</li></settlement></list><tree><country name="République populaire de Chine"><noRegion><name sortKey="Jun Liu" sort="Jun Liu" uniqKey="Jun Liu" last="Jun Liu">JUN LIU</name></noRegion><name sortKey="Feng Gao" sort="Feng Gao" uniqKey="Feng Gao" last="Feng Gao">FENG GAO</name><name sortKey="Fuliang Chu" sort="Fuliang Chu" uniqKey="Fuliang Chu" last="Fuliang Chu">FULIANG CHU</name><name sortKey="Gao, George F" sort="Gao, George F" uniqKey="Gao G" first="George F." last="Gao">George F. Gao</name><name sortKey="Gao, George F" sort="Gao, George F" uniqKey="Gao G" first="George F." last="Gao">George F. Gao</name><name sortKey="Gao, George F" sort="Gao, George F" uniqKey="Gao G" first="George F." last="Gao">George F. Gao</name><name sortKey="Gao, George F" sort="Gao, George F" uniqKey="Gao G" first="George F." last="Gao">George F. Gao</name><name sortKey="Hao Wu" sort="Hao Wu" uniqKey="Hao Wu" last="Hao Wu">HAO WU</name><name sortKey="Jianxun Qi" sort="Jianxun Qi" uniqKey="Jianxun Qi" last="Jianxun Qi">JIANXUN QI</name><name sortKey="Jianxun Qi" sort="Jianxun Qi" uniqKey="Jianxun Qi" last="Jianxun Qi">JIANXUN QI</name><name sortKey="Jinghua Yan" sort="Jinghua Yan" uniqKey="Jinghua Yan" last="Jinghua Yan">JINGHUA YAN</name><name sortKey="Jinghua Yan" sort="Jinghua Yan" uniqKey="Jinghua Yan" last="Jinghua Yan">JINGHUA YAN</name><name sortKey="Jun Liu" sort="Jun Liu" uniqKey="Jun Liu" last="Jun Liu">JUN LIU</name><name sortKey="Jun Liu" sort="Jun Liu" uniqKey="Jun Liu" last="Jun Liu">JUN LIU</name><name sortKey="Taisheng Li" sort="Taisheng Li" uniqKey="Taisheng Li" last="Taisheng Li">TAISHENG LI</name><name sortKey="Yeping Sun" sort="Yeping Sun" uniqKey="Yeping Sun" last="Yeping Sun">YEPING SUN</name><name sortKey="Yeping Sun" sort="Yeping Sun" uniqKey="Yeping Sun" last="Yeping Sun">YEPING SUN</name><name sortKey="Yeping Sun" sort="Yeping Sun" uniqKey="Yeping Sun" last="Yeping Sun">YEPING SUN</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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|texte= The Membrane Protein of Severe Acute Respiratory Syndrome Coronavirus Acts as a Dominant Immunogen Revealed by a Clustering Region of Novel Functionally and Structurally Defined Cytotoxic T-Lymphocyte Epitopes
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